Understanding glycans and glycoproteins at the cell surface for cardiovascular diseases, by Rebekah Gundry, Professor and Vice-Chair, Director, CardiOmics Program, College of Medicine Dept of Cellular & Integrative Physiology, University of Nebraska

Rebekah Gundry, Professor and Vice-Chair, Director, CardiOmics Program, College of Medicine Dept of Cellular & Integrative Physi
Rebekah Gundry, Professor and Vice-Chair, Director, CardiOmics Program, College of Medicine Dept of Cellular & Integrative Physiology, University of Nebraska

 The Department of Chemistry Presents, via Online Zoom Presentation:   by Rebekah Gundry, Professor and Vice-Chair, Director, CardiOmics Program, College of Medicine Dept of Cellular & Integrative Physiology, University of Nebraska

 

Cell surface glycoproteins and glycans play critical roles in a range of biological functions and disease processes and may be exploited as biomarkers for precision medicine. Despite their biological relevance and utility, glycoproteins and glycans are often understudied largely due to technical challenges. This presentation will describe our recently developed analytical and bioinformatic solutions that enable rapid identification and quantification of cell surface glycoproteins and glycans from small sample sizes. The application of these new methodologies to address outstanding questions in cardiac biology and disease, with an emphasis on precision medicine, will be described.

The Gundry laboratory develops and applies innovative mass spectrometry (MS) technologies, bioinformatics tools, and methodologies to transform our understanding of cell surface proteins and glycans and answer outstanding questions in stem cell biology and cardiac pathology. Our platforms promote the development of new reagents and strategies to 1) improve the quality and homogeneity of stem cell-derived cardiomyocytes for research and clinical applications, and 2) promote the discovery of therapeutic and monitoring strategies for advanced heart failure.

At the heart of our research program is the tenet that developing innovative technologies and bioinformatic tools will enable us to answer questions that cannot be addressed by current methodologies and will lead to fundamental discoveries that support mechanistic research. We develop and apply innovative MS approaches to identify, characterize, and quantify glycoproteins and glycans in hPSC-CM and primary heart cells. We focus on the subset of molecules localized to the cell surface (i.e. the surfaceome), including transmembrane, GPI-anchored, and ECM proteins and glycans because these can be optimally exploited for immunophenotyping, drug targeting, and are critical players in normal cardiac function and disease.

Working at the interface of analytical chemistry, stem cell biology and cardiac biology, the major tools in our toolbox include protein biochemistry, mass spectrometry, proteomics, metabolomics, glycoproteomics, glycomics, chromatography, immunofluorescence imaging, flow cytometry, and state-of-the art stem cell culturing and differentiation methods.

 

Bio

Dr. Gundry is Professor and Vice Chair of Cellular and Integrative Physiology, Assistant Chief of Basic and Translational Research for the Division of Cardiology and is the Director of the CardiOmics Program at the University of Nebraska Medical Center. The Gundry lab applies mass spectrometry technologies for advancing basic and translational cardiac research and clinical care. Through development and application of innovative approaches and bioinformatic tools to study cell surface glycoproteins and glycans, the Gundry lab aims to answer outstanding questions in stem cell and cardiac biology and disease. Dr. Gundry is a Council Member of HUPO, Co-Chair of the HUPO B/D HPP Cardiovascular Initiative, is on the Board of Directors of US HUPO, and is an Associate Editor of the Journal of Molecular and Cellular Cardiology. The Gundry lab is funded by the American Heart Association, Juvenile Diabetes Research Foundation, and National Institutes of Health.

Research Group Website: https://www.gundrylab.com/

 

This will be an Online Zoom Seminar.