Erik A. Rodriguez
Areas of Expertise
Fluorescent Proteins, Fluorescence Imaging, Bioengineering, Biochemistry, Biophysics, Chemical Biology, Biosensors, Imaging Probes
Throughout my doctoral and post-doctoral training, I have pursued research in biological and chemical tool development to elucidate biology.
In my doctoral work at Caltech with Dennis A. Dougherty and Henry A. Lester, I developed orthogonal suppressor tRNAs to site-specifically incorporate multiple unnatural amino acids in vivo. I received broad training in chemical biology, biochemistry, and neurobiology.
My postdoctoral work at UCSD with Roger Y. Tsien enhanced my expertise to include evolving and characterizing fluorescent proteins, fluorescence imaging, cell culture, and animal imaging. I enjoy collaborating to gain knowledge in diverse fields and to ensure my tools are utilized. The newly evolved fluorescent protein is biophysically the brightest far-red fluorescent protein created, so far, and comparable to jellyfish derived fluorescent proteins.
Richard Ting (Weill Cornell Medicine) and I developed dual-modality imaging (near-infrared fluorescence and positron emission tomography) agents to image cancer, strokes, and cerebrospinal fluid in living mice, which will be tested in humans.
My lab will continue to develop new fluorescent proteins, protein labeling tags, and tools for imaging human maladies.
I will evolve new fluorescent proteins with unique properties compared to currently available fluorescent proteins. New biosensors will be created and characterized for sensing calcium, cAMP, voltage, and other molecules or chemical properties of the cell. Improving genetically encoded, photoacoustic imaging probes will allow for deeper imaging than single photon fluorescence for non-invasive optical imaging in living animals. A far-red and near-infrared fluorescence cell cycle indicator has been developed and biological applications in cancer will be explored.
Richard Ting (Weill Cornell Medicine) and I will continue to develop probes. The new fluorescent protein was evolved to express in large quantities (grams are easily purified in <24 hours). The protein is an excellent scaffold or nanocarrier for attachment of chemical molecules to enhance signal to noise for positron emission tomography and magnetic resonance imaging. Additionally, cancer drugs can be attached for drug delivery and location is verified with imaging.
Postdoctoral Scholar, University of California, San Diego, Professor Roger Y. Tsien (2008 Nobel Laureate)
Ph.D., California Institute of Technology (Caltech), Professor Dennis A. Dougherty,2009
B.S., California Institute of Technlogy (Caltech), 2002
Sadegh, S., Yang, MH., Ferri, CGL., Thunemann, M., Saisain, PA., Wei, Z., Rodriguez, EA., Adams, SR., Kilic, Kivilcim, Boas, DA., Sakadzic, S., Devor, A., & Fainman, Y.. 2019 “Efficient non-degenerate two-photon excitation for fluorescence microscopy.” Under review.
Mo, GCH., Posner, C., Rodriguez, EA., & Zhang, J.. 2019 “Rationally enhanced red fluorescent protein expands the utility of FRET biosensors.” Under review.
Maiti, A., Buffalo, C., Saurabh, S., Hachey, J., Tran, GN., Drobizhev, M., Hughes, TE., Moerner, WE., Ghosh, P., Matsuo, H., Tsien, RY., Lin, JY., & Rodriguez, EA.. 2019 “Crystal structure and biophysical characterization of the small ultra-red fluorescent protein evolved from a phycobiliprotein.” Submitted.
Machado, JH., Lin, JY., Ting, R., & Rodriguez EA.. 2019 “A self-labeling protein based on the small ultra-red fluorescent protein.” In preparation for submission.
Chen, N., An, F., Guo, H., Kommidi, H., Yang, X., Rodriguez, EA., & Ting, R.. 2019 “pH paper for cells: a ratiometric fluorescent probe using a modified far-red fluorescent protein nanosensor.” In preparation for submission.
Guo, H., Kommidi, H., Vedvyas, Y., McCloskey, JE., Zhang, J., Chen, N., Nurili, F., Wu, AP., Sayman, H., Akin, O., Rodriguez, EA., Aras, O., Jin, MM., & Ting, R.. 2019 “A fluorescent, [18F]-positron-emitting agent for imaging PMSA allows genetic reporting in adoptively-transferred, genetically-modified cells.” American Chemical Society Chemical Biology. 14(7): 1449-59.
Almogbil, H., Daszynski, C., Rodriguez, EA., Singh, T., Stepp, MA., & Zderic, V.. 2019 “Therapeutic ultrasound for improving the topical corneal delivery of macromolecules.” The Journal of the Acoustical Society of America. 145(3): 1894.
Smith, M., Burgos, M., & McCoy, F.. 2019 Science of the Sea: A Hawai‘i-Based Perspective of Oceanography. Chapter 5 Chemistry of the Ocean. Pueo Press. ISBN-10: 179843038X. Turtle image, made with E. coli expressing CFP and mTangerine, is featured at the start of Ch. 5, drawn by Rodriguez, EA..
Rodriguez, EA., Tran, GN., Gross, LA., Crisp, JC., Shu, XS., Lin, JY., & Tsien, RY.. 2016 “A far-red fluorescent protein evolved from a cyanobacterial phycobiliprotein.”Nature Methods. 13(9): 763-9.
Rodriguez, EA., Wang, Y., Crisp, JL., Vera, DR., Tsien, RY., & Ting, R.. 2016 “New dioxaborolane chemistry enables 18F-positron-emitting, fluorescent 18F-multimodality biomolecule generation from the solid phase." Bioconjugate Chemistry. 27(5): 1390-9.
Rodriguez, EA., Campbell, RE., Lin, JY., Lin, MZ., Miyawaki, A., Palmer, AE., Shu, X., Zhang, J., & Tsien, RY.. 2017 “The growing and glowing toolbox of fluorescent and photoactive proteins.” Trends in Biochemical Sciences. 42(2): 111-29.
Wang, Y., An, FF., Chan, M., Friedman, B., Rodriguez, EA., Tsien, RY., Aras, O., & Ting, R.. 2017 “18F-positron-emitting/fluorescent labeled erythrocytes allow imaging of internal hemorrhage in murine intracranial hemorrhage model.” Journal of Cerebral Blood Flow and Metabolism. 37(3): 776-86.
Pantoja, R., Rodriguez, EA., Dibas, MI., Dougherty, DA., & Lester, HA.. 2009 “Single-molecule imaging of a fluorescent unnatural amino acid incorporated into nicotinic receptors.” Biophysical Journal. 96(1): 226-37.
Rodriguez, EA., Lester, HA., & Dougherty, DA.. 2007 “Improved amber and opal suppressor tRNAs for incorporation of unnatural amino acids in vivo. Part 1: Minimizing misacylation.” RNA 13: 1703- 14.
Rodriguez, EA., Lester, HA., & Dougherty, DA.. 2007 “Improved amber and opal suppressor tRNAs for incorporation of unnatural amino acids in vivo. Part 2: Evaluating suppression efficiency.” RNA 13: 1715-22.
Rodriguez, EA., Lester, HA., & Dougherty, DA.. 2006 “In vivo incorporation of multiple unnatural amino acids through nonsense and frameshift suppression.” Direct submission to the Proceedings of the National Academy of Sciences of the United States of America. 103(23): 8650-5.
Samson, AO., Chill, JH., Rodriguez, E., Scherf, T., & Anglister, J.. 2001 “NMR mapping and secondary structure determination of the major acetylcholine receptor α-subunit determinant interacting with α-bungarotoxin.” Biochemistry. 40(18): 5464-73.
We collaborate with the GW Department of Biomedical Engineering, GW Cancer Center (Erik A. Rodriguez is a member), the National Institutes of Health, and many labs around the world, including W.E. Moerner (Stanford, 2014 Nobel laureate), Robert Campbell (University of Alberta & University of Tokyo), Roger Zemp (University of Alberta), Jin Zhang (University of California, San Diego), Anna Devor (University of California, San Diego), Hiroshi Matsuo (NIH, NCI), Richard Ting (Weill Cornell Medicine), John Lin (University of Tasmania), Vesna Zderic (GW SEAS, Biomedical Engineering), and Mary Ann Stepp (GW SMHS, Anatomy and Cell Biology and Ophthalmology). We are always open to new collaborations and feel free to contact us.
CHEM 4123: Instrumental Analytical Chemistry Laboratory
CHEM 6370: Biophysical Chemistry