Dr. Ling Hao

Areas of Expertise

Bioanalytical Chemistry, Mass Spectrometry, Proteomics, Metabolomics, Neurodegenerative Diseases

We are hiring!

If you are interested in joining our lab as graduate/undergraduate/visiting students or postdoc fellows, please email me attaching your CV.

Current Research

Quantitative analysis of biomolecules offers a wealth of clues to understand cell biology and disease mechanisms. Mass spectrometry (MS) has become a central technology to study biomolecules such as proteins, peptides, lipids, and metabolites. Our research focuses on developing novel and improved bioanalytical methods using LC-MS platforms and applying the combination of analytical chemistry, bioinformatics, and cell biology approaches to study human diseases. Specifically, 1) we develop novel proteomics and metabolomics strategies in complex biological samples; 2) we implement multidisciplinary approaches in the human induced pluripotent stem cell (iPSC)-derived neurons, mouse models, and clinical human subjects to study the molecular mechanisms of neurodegenerative diseases. 

Education

Postdoc, National Institutes of Health, 2019

PhD, University of Wisconsin-Madison, 2017

BS, China Agricultural University, 2012 Tsinghua University, 2011-2012

Publications

Frankenfield, A.M., Fernandopulle, M.S., Hasan, S., Ward, M.E. and Hao, L., “Development and Comparative Evaluation of Endolysosomal Proximity Labeling-based Proteomic Methods in Human iPSC-derived Neurons”. Analytical Chemistry, 2020.

Y. Liao, M. Fernandopulle, G. Wang, H. Choi, L. Hao, C. Drerup, S. Qamar, J. Abell, et al… A. Pasolli, L. Forrest, P. George-Hyslop, J. Lippincott-Schwartz, M. Ward. “RNA granules hitchhike on lysosomes for long-distance transport, using annexin A11 as a molecular tether.” Cell, 179(1), pp.147-164, 2019.

Hao, L., Zhu, Y., Wei, P., Johnson, J., Buchberger, A., Frost, D., Kao, W.J. and Li, L., “Metandem: An online software tool for mass spectrometry-based isobaric labeling metabolomics”. Analytica Chimica Acta, 1088, pp.99-106, 2019.

D. Sliter, J. Martinez, L. Hao, X. Chen, N. Sun, T. Fischer, J. Burman, Y. Li, Z. Zhang, D. Narendra, H. Cai, C. Klein, R. Youle. “Parkin and PINK1 Mitigate STING-induced Inflammation.” Nature, 561,258, 2018.

L. Hao, J. Wang, D. Page, S. Asthana, H. Zetterberg, C. Carlsson, O. Okonkwo, L. Li. “Comparative Evaluation of MS-based Metabolomics Software and the Application to Preclinical Alzheimer’s Disease.” Scientific Reports, 8, 9291, 2018.

L. Hao, J. Johnson, C. Lietz, A. Buchberger, D. Frost, W. Kao, L. Li. “Mass Defect-based Dimethyl Leucine Labels for Quantitative Proteomics and Amine Metabolomics of Pancreatic Cancer Cells.” Analytical Chemistry, 89, 1138, 2017. (cover article)

J. Lu, X. Zhong, H. Liu, L. Hao, C. Huang, M. Sherafat, J. Jones, L. Li, S. Zhang. “Generation of Serotonin Neurons from Human Pluripotent Stem Cells.” Nature Biotechnology, 34, 89, 2016.

S. Thomas, L. Hao, W. Ricke, L. Li. “Biomarker Discovery in Mass Spectrometry-based Urinary Proteomics.” PROTEOMICS-Clinical Applications, 10, 358, 2016. (Cover article)

Classes Taught

Chem4122: Instrumental Analytical Chemistry

Chem6222: Biomedical Mass Spectrometry

Research Courses: 8998, 6395, 4195