Quantitative analysis of biomolecules offers a wealth of clues to understand cell biology and disease mechanisms. Mass spectrometry (MS) has become a central technology to study biomolecules such as proteins, peptides, lipids, and metabolites. Our research focuses on developing novel and improved bioanalytical methods using LC-MS platforms and applying the combination of analytical chemistry, bioinformatics, and cell biology approaches to study human diseases. Specifically, 1) we develop novel proteomics and metabolomics strategies in complex biological samples; 2) we implement multidisciplinary approaches in the human induced pluripotent stem cell (iPSC)-derived neurons, mouse models, and clinical human subjects to study the molecular mechanisms of neurodegenerative diseases.
Li, H., Frankenfield, A.M., Houston, R., Sekine, S., and Hao, L., “Thiol-Cleavable Biotin for Chemical and Enzymatic Biotinylation and Its Application to Mitochondrial TurboID Proteomics”. Journal of the American Society for Mass Spectrometry, 2021. (Invited for Emerging Investigators Focus Issue)
Li, H., Uittenbogaard, M., Hao, L., and Chiaramello, A., “Clinical Insights into Mitochondrial Neurodevelopmental and Neurodegenerative Disorders: Their Biosignatures from Mass Spectrometry-Based Metabolomics”. Metabolites, 11 (4), 2021.
Frankenfield, A.M., Fernandopulle, M.S., Hasan, S., Ward, M.E. and Hao, L., “Development and Comparative Evaluation of Endolysosomal Proximity Labeling-based Proteomic Methods in Human iPSC-derived Neurons”. Analytical Chemistry, 92 (23), 2020.
Liao, Y.C., Fernandopulle, M.S., Wang, G., Choi, H., Hao, L., Drerup, C.M., Patel, R., Qamar, S., Nixon-Abell, J., et al. Pasolli, A., Forrest, L., George-Hyslop, P., Lippincott-Schwartz, J., Ward, M., “RNA granules hitchhike on lysosomes for long-distance transport, using annexin A11 as a molecular tether.” Cell, 179(1), 2019.
Hao, L., Zhu, Y., Wei, P., Johnson, J., Buchberger, A., Frost, D., Kao, W.J. and Li, L., “Metandem: An online software tool for mass spectrometry-based isobaric labeling metabolomics”. Analytica Chimica Acta, 1088, 2019.
Hao, L., Wang, J., Page, D., Asthana, S., Zetterberg, H., Carlsson, C., … & Li, L. “Comparative Evaluation of MS-based Metabolomics Software and Its Application to Preclinical Alzheimer’s Disease”. Scientific Reports, 8, 2018.
Hao, L., Johnson, J., Lietz, C.B., Buchberger, A., Frost, D., Kao, W.J. and Li, L., “Mass defect-based N, N-dimethyl leucine labels for quantitative proteomics and amine metabolomics of pancreatic cancer cells”. Analytical Chemistry, 89(2), 2017. (Cover article)
Lu, J., Zhong, X., Liu, H., Hao, L., Huang, C.T., Sherafat, M.A., Jones, J., Ayala, M., Li, L. and Zhang, S.C., “Generation of serotonin neurons from human pluripotent stem cells.” Nature Biotechnology, 34(1), p.89. 2016.