Dr. Ling Hao

Areas of Expertise

Bioanalytical Chemistry, Mass Spectrometry, Proteomics, Metabolomics, Neurodegenerative Diseases

We are hiring!

If you are interested in joining our lab as graduate/undergraduate/visiting students or postdoc fellows, please email me attaching your CV.

Current Research

Quantitative analysis of biomolecules offers a wealth of clues to understand cell biology and disease mechanisms. Mass spectrometry (MS) has become a central technology to study biomolecules such as proteins, peptides, lipids, and metabolites. Our research focuses on developing novel and improved bioanalytical methods using LC-MS platforms and applying the combination of analytical chemistry, bioinformatics, and cell biology approaches to study human diseases. Specifically, 1) we develop novel proteomics and metabolomics strategies in complex biological samples; 2) we implement multidisciplinary approaches in the human induced pluripotent stem cell (iPSC)-derived neurons, mouse models, and clinical human subjects to study the molecular mechanisms of neurodegenerative diseases. 

Education

Postdoc, National Institutes of Health, 2019

PhD, University of Wisconsin-Madison, 2017

BS, China Agricultural University, 2012 Tsinghua University, 2011-2012

Publications

• Li, H., Frankenfield, A.M., Houston, R., Sekine, S., and Hao, L., “Thiol-Cleavable Biotin for Chemical and Enzymatic Biotinylation and Its Application to Mitochondrial TurboID Proteomics”. Journal of the American Society for Mass Spectrometry, 2021. (Invited for Emerging Investigators Focus Issue)
• Li, H., Uittenbogaard, M., Hao, L., and Chiaramello, A., “Clinical Insights into Mitochondrial Neurodevelopmental and Neurodegenerative Disorders: Their Biosignatures from Mass Spectrometry-Based Metabolomics”. Metabolites, 11 (4), 2021.
• Frankenfield, A.M., Fernandopulle, M.S., Hasan, S., Ward, M.E. and Hao, L., “Development and Comparative Evaluation of Endolysosomal Proximity Labeling-based Proteomic Methods in Human iPSC-derived Neurons”. Analytical Chemistry, 92 (23), 2020.
• Liao, Y.C., Fernandopulle, M.S., Wang, G., Choi, H., Hao, L., Drerup, C.M., Patel, R., Qamar, S., Nixon-Abell, J., et al. Pasolli, A., Forrest, L., George-Hyslop, P., Lippincott-Schwartz, J., Ward, M., “RNA granules hitchhike on lysosomes for long-distance transport, using annexin A11 as a molecular tether.” Cell, 179(1), 2019.
• Hao, L., Zhu, Y., Wei, P., Johnson, J., Buchberger, A., Frost, D., Kao, W.J. and Li, L., “Metandem: An online software tool for mass spectrometry-based isobaric labeling metabolomics”. Analytica Chimica Acta, 1088, 2019.
• Sliter, D.A., Martinez, J., Hao, L., Chen, X., Sun, N., Fischer, T.D., Burman, J.L., Li, Y., Zhang, Z., Narendra, D.P. and Cai, H., Borsche, M., Klein, C., Youle., R.,”Parkin and PINK1 mitigate STING-induced inflammation”. Nature, 561(7722), 2018.
• Hao, L., Wang, J., Page, D., Asthana, S., Zetterberg, H., Carlsson, C., … & Li, L. “Comparative Evaluation of MS-based Metabolomics Software and Its Application to Preclinical Alzheimer’s Disease”. Scientific Reports, 8, 2018.
• Hao, L., Johnson, J., Lietz, C.B., Buchberger, A., Frost, D., Kao, W.J. and Li, L., “Mass defect-based N, N-dimethyl leucine labels for quantitative proteomics and amine metabolomics of pancreatic cancer cells”. Analytical Chemistry, 89(2), 2017. (Cover article)
• Lu, J., Zhong, X., Liu, H., Hao, L., Huang, C.T., Sherafat, M.A., Jones, J., Ayala, M., Li, L. and Zhang, S.C., “Generation of serotonin neurons from human pluripotent stem cells.” Nature Biotechnology, 34(1), p.89. 2016.

Classes Taught

CHEM 4122: Instrumental Analytical Chemistry

CHEM 6222: Biomedical Mass Spectrometry

CHEM 6284: Environmental Analytical Chemistry

CHEM 2123W Introductory Quantitative Analysis Laboratory

Research Courses: 8998, 6395, 4195